Composite

Part:BBa_K2041021:Design

Designed by: YANG YUANZHAN   Group: iGEM16_BIT   (2016-10-19)


promoter-RBS-binding site of miR-155-GFP


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 7
    Illegal NheI site found at 30
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 61
    Illegal BsaI.rc site found at 831


Design Notes

This is a composite part including the binding site of miR-155 and GFP coding region.


Source

The promoter and GFP are from Kit Plate of iGEM foundation and the binding site of miR-155 is synthesised by SinoGeno Max Co. Ltd.

References